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. 2021 Sep 22;11:18849. doi: 10.1038/s41598-021-98086-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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CD177 cell surface level expression is higher in ACLF versus CLD. (A) Gating strategy for assessment of CD17⁺ cells by flow cytometry. Neutrophil enrichment was determined by CD66b⁺ and CD16⁺positivity. The first plot represents an FSC versus SSC scatter plot, used for gating PMN cells. Singlets gating as determined by FSC-height v/s FSC-area plot. This is followed by a quadrant plot for CD66b-FITC versus CD16-APC, and double positive neutrophils are gated on Q2. The last quadrant plot represents CD177-APC-Cy7 versus CD16-APC. CD177 positive cells are gated. (B) CD66b⁺CD16⁺ positive neutrophil percentage across groups. The mean percentage of enrichment is significantly different across groups as determined by Kruskal Wallis test (C) CD177 positive cell percentage across study groups. The mean CD177 population was found to be significantly different across the three groups, as calculated by one-way ANOVA. Pairwise analysis for CD177 + cell percentages were performed for ACLF versus CLD. *p value ≤ 0.05; ** p value ≤ 0.005, ***p value ≤ 0.001.