Table 1.
Summary of nanobody binding kinetics.
| Analyte | Ligand | Ka (1/Ms) | Kd (1/s) | KD (pM) | T1/2 (min) |
|---|---|---|---|---|---|
| C1 | RBD | 9.3E + 05 | 5.7E-04 | 615 | 20 |
| C1 | Alpha RBD | 7.5E + 05 | 5.4E-04 | 725 | 21 |
| C1 | Beta RBD | 9.2E + 05 | 6.0E-04 | 648 | 19 |
| C5 | RBD | 9.8E + 06 | 9.8E-04 | 99 | 12 |
| C5 | Alpha RBD | 6.8E + 06 | 1.7E-02 | 2523 | 1 |
| H3 | RBD | 1.3E + 07 | 3.3E-04 | 25 | 35 |
| H3 | Alpha RBD | 1.2E + 07 | 1.2E-03 | 102 | 10 |
| F2 | RBD | 4.7E + 06 | 1.9E-04 | 40 | 61 |
| F2 | Alpha RBD | 4.8E + 06 | 2.3E-04 | 47 | 51 |
| F2 | Beta RBD | 5.9E + 06 | 2.2E-04 | 38 | 52 |
| C5 Fc | RBD | 3.1E + 06 | 1.2E-04 | 37 | 99 |
| C5 trimer | RBD-Fc | 7.1E + 06 | 1.2E-04 | 18 | 92 |
| C5 trimer | Alpha RBD-Fc | 9.9E + 06 | 2.8E-04 | 29 | 41 |
| H3 trimer | RBD-Fc | 1.2E + 08 | 3.3E-05 | 0.3 | 349 |
| H3 trimer | Alpha RBD-Fc | 1.8E + 07 | 1.2E-04 | 6 | 98 |
| C1 trimer | RBD-Fc | 9.0E + 05 | 4.8E-05 | 53 | 242 |
| C1 trimer | Alpha RBD-Fc | 1.0E + 06 | 7.4E-05 | 73 | 154 |
| C1 trimer | Beta RBD-Fc | 8.2E + 05 | 6.2E-05 | 75 | 186 |