Table 3.
EM statistics.
| Spike C5 (PDB ID 7OAN, EMD-12777) | |
|---|---|
| Data collection and processing | |
| Magnification | 81,000 |
| Voltage (kV) | 300 |
| Electron exposure (e–/Å2) | 50 |
| Defocus range (μm) | 1.0–3.0 |
| Pixel size (Å/pix) (super resolution) | 0.53 |
| Symmetry imposed | C3 |
| Initial particle images (no.) | 1,061,364 |
| Final particle images (no.) | 227,898 |
| Map resolution (Å) | 2.9 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 2.7–6.7 |
| Refinement | |
| Initial model used | 6VXX |
| Model resolution (Å) | 3.0 |
| FSC threshold | 0.143 |
| Model resolution range (Å) | 198.2–3.0 |
| Map sharpening B factor (Å2) | −118 |
| Model composition | |
| Non-hydrogen atoms | 28,218 |
| Protein residues | 3510 |
| B factors (Å2) | |
| Protein | 121 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.011 |
| Bond angles (°) | 1.241 |
| Validation | |
| MolProbity score | 1.84 |
| Clashscore | 8.13 |
| Poor rotamers (%) | 1.35 |
| Ramachandran plot | |
| Favoured (%) | 95.75 |
| Allowed (%) | 4.08 |
| Disallowed (%) | 0.17 |