FIG. 5.

Ku is associated with the Ty1 cDNA and integrase. (A) Immunoprecipitations were performed with a polyclonal antibody directed against Yku70p. These were done with VLPs prepared from the wild-type strain containing VLPs (W303 + pGTy1, lanes 3 and 4), the yku70 mutant strain (yku70 + pGTy1, lanes 5 and 6), or analogous fractions from the wild-type strain without pGTy1 (W303, lanes 1 and 2). DNA was isolated from the I and P fractions and analyzed by PCR using primers that amplify a portion of pGTy1. PCR products are shown from reactions containing pGTy1 (lane 7) or no DNA (lane 8). (B) Western blot analysis of the I, supernatant (S), and P fractions of the immunoprecipitations performed as described for panel A using monoclonal antibody 8B11 (20) directed against the integrase (IN) protein.