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. 2021 Sep 22;7:260. doi: 10.1038/s41420-021-00633-7

Fig. 2. ZGRF1 deletion impairs HR repair.

Fig. 2

A ZGRF1 depletion inhibits the efficiency of DNA damage repair as shown by the increased residual γH2AX foci in HeLa cells. B Quantification of γH2AX foci in the HeLa cells at different time after 4 Gy irradiation. Data are means ± SD from three independent experiments (50 cells were scored in each experiment). *P < 0.05; **P ≤ 0.01, two-tailed Student’s t test. C HR efficiency was determined using the direct repeat GFP (DR-GFP) reporter assay. D The NHEJ efficiency was determined using the EJ5-GFP reporter assay. BRCA1 and 53BP1 siRNAs were used as a positive or negative control, respectively. Data are means ± SD from three independent experiments. *P < 0.05; **P ≤ 0.01. two-tailed Student’s t-test. E Knocking down efficiency of the indicated siRNA were detected by western blotting. F–H ZGRF1 deletion sensitizes HeLa (F) and MD231 (G) cancer cells to the PARP inhibitor olaparib, measured by colony formation assay. SR50 (H) represents the concentration for 50% cell survival. Data are means ± SEM from three independent experiments. *P < 0.05; **P ≤ 0.01. Two-way analysis of variance (ANOVA).