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. 2021 Sep 22;11:18856. doi: 10.1038/s41598-021-98412-y

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Prolonged perilymphatic leakage causes hearing loss. (a) ABR thresholds of adult mice undergoing PSC fenestration. Some mice developed hearing loss after PSC opening while others did not. (b) ABR thresholds of adult mice undergoing PSC fenestration, controlling for perilymphatic leakage time. Mice with 10-min PSC opening time exhibited significant ABR threshold elevations across all frequencies compared with non-surgery control mice (one-way ANOVA). Data are represented as mean ± SEM. n represents the number of animals tested. Statistical significance between groups is shown above error bars (*represents p < 0.05, **represents p < 0.01, ***represents p < 0.001).