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. 2021 Sep 22;11:18789. doi: 10.1038/s41598-021-98226-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Detailed visualization of alignment in the recombination region of M. bovis dataset affecting the narX gene encoding a probable nitrate reductase. No gaps or undefined nucleotides were identified in the recombination region of internal nodes. This specific event was registered across Eu1 genomes. The quality of sequencing of narX gene was evaluated by read mapping against M. bovis AF2122/97. The SNP positions suggested in the recombination region were confirmed by applying the criteria referred to in the methods section (at least 20 reads and 0.9 frequency of alteration). The polymorphisms at narX gene were fully confirmed in genomes Mb1792361 and Mb7240415 (2.3%).