Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 22;12:5578. doi: 10.1038/s41467-021-25792-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a Heatmap showing the expression of cone and ganglion markers in retinal organoids at different differentiation time points, and in subtype 1 and subtype 2 tumors assessed by NanoString technology. Differences in gene expression between the two subtypes were assessed by two-sided t-tests with BH correction. Exact p-values are provided in Supplementary Data 4. b Pearson’s correlation of the expression of 8 cone markers, between the centroids of the 2 retinoblastoma subtypes and retinal organoids at different time points in differentiation. C1: centroid of subtype 1; C2: centroid of subtype 2. c Phylogenetic tree based on cone marker expression, for retinal organoids at different differentiation time points and for retinoblastoma samples. d Immunohistochemical staining of CRX, ARR3, EBF3, and Ki-67 in normal retina and retinoblastoma. For RB617, the black arrows indicate the mutually exclusive patterns for ARR3 and EBF3. Immunohistochemistry experiments were performed on 34 samples (subtype 1, n = 9; subtype 2, n = 25). Two representative images are shown for each subtype. e Boxplots showing the quick score (QS) for the differentiation markers used in the immunohistochemical analysis: CRX, ARR3, and EBF3. In the boxplots, the central mark indicates the median and the bottom and top edges of the box the 25th and 75th percentiles. The whiskers are the smaller of 1.5 times the interquartile range or the length of the 25th percentiles to the smallest data point or the 75th percentiles to the largest data point. Data points outside the whiskers are outliers. Two-sided Wilcoxon tests were used.