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. 2021 Sep 22;12(10):858. doi: 10.1038/s41419-021-04150-4

Fig. 4. Inhibition of CAFs autophagy attenuates their regulation on EMT and metastasis-related genes of lung cancer cells.

Fig. 4

A–C CAFs were treated with 3-MA (5 mM) or CQ (60 µM) for 2 h or transfected with siATG5 for 24 h. Medium was replaced with fresh medium and CAF-CM was collected after 24 h, and added to lung cancer cells. The mRNA and protein expressions were detected by qPCR and western blotting. D–F CAFs were treated with 3-MA (5 mM) or CQ (60 µM) for 2 h or transfected with siATG5 for 24 h. CAF-CM was collected and added to lung cancer cells. The mRNA and protein expressions were detected by qPCR and western blotting. G CAFs were treated with RAPA (100 nM) for 2 h. CAF-CM was collected and added to lung cancer cells. The gene expressions were detected by western blotting. Data represent the mean ± SD from three independent experiments. Columns, mean; bars, SD. *p < 0.05, **p < 0.01, ***p < 0.001. Pre-Tx pretreatment.