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. 2021 Sep 22;12(10):858. doi: 10.1038/s41419-021-04150-4

Fig. 5. CAFs autophagy activity regulates HMGB1 secretion.

Fig. 5

A The expressions of HMGB1 in paired CAFs and NFs were detected by western blotting (n = 3). B HMGB1 released by paired CAFs and NFs or lung cancer cells were detected by ELISA (n = 3). C CAFs were treated with 3-MA (5 mM) for 2 h or transfected with siATG5 for 24 h. The expressions of HMGB1 were detected by western blotting. D CAFs were treated with 3-MA (5 mM) or CQ (60 µM) for 2 h or transfected with siATG5 for 24 h. HMGB1 released by CAFs was detected by ELISA. E CAFs were treated with RAPA (100 nM) for 2 h. HMGB1 released by CAFs was detected by ELISA. F CAFs were treated with recombinant HMGB1 (50 ng/mL) for 48 h. The LC3 puncta patterns were observed under a confocal microscope. Scale bar, 10 μm. G CAFs were treated with recombinant HMGB1 (50 ng/mL) for 48 h. The expression of ATG5 and LC3 was detected by western blotting. Data represent the mean ± SD from three independent experiments. H, I HMGB1 expression level in LUCA, and LUCA patient survival. Columns, mean; bars, SD. *p < 0.05, **p < 0.01, ***p < 0.001. Pre-Tx pretreatment.