Fig. 7. Autophagy activity is responsible for CAFs’ modulation on TLR4/NF-κB pathway in lung cancer cells via HMGB1 secretion.

A CAFs were treated with 3-MA (5 mM) or CQ (60 µM) for 2 h. Medium was replaced with fresh medium and CAF-CM was collected after 24 h, and added to lung cancer cells. The gene expressions were detected by western blotting. B CAFs were treated with 3-MA (5 mM) or CQ (60 µM) for 2 h. CAF-CM was collected and added to lung cancer cells. The expressions of p65 in nuclear compartment and cytoplasmic compartment were detected by western blotting. C, D CAFs were transfected with siATG5 or treated with RAPA (100 nM). CAF-CM was collected and added to lung cancer cells. The gene expressions were detected by western blotting. E, F Recombinant HMGB1 (50 ng/mL) or HMGB1 neutralizing antibody was added to culture medium. The gene expressions were detected by western blotting.