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. 2021 Sep 22;11:18802. doi: 10.1038/s41598-021-98322-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Knockdown of PAX3-FOXO1 downregulates the expression of B7-H3. (a) Quantitative real-time PCR (qRT-PCR) analysis of PAX3-FOXO1. Knockdown efficacy of PAX3-FOXO1 was confirmed in three PAX3-FOXO1 positive ARMS cell lines. RD, a PAX3-FOXO1-negative cell line, was used as a negative control. (b) qRT-PCR analysis of B7-H3. (c) Representative histogram of flow cytometry analysis of B7-H3. (d) Mean fluorescence index of B7-H3 in flow cytometry analysis. Results shown are means ± SD. *p < 0.05, **p < 0.01, n.s. p > 0.05, by two-tailed unpaired t test.