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. 2021 Sep 22;4:1117. doi: 10.1038/s42003-021-02595-z

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a UMAP representation of the relative expression of selected markers analyzed by flow cytometry by manually gated CD8+ CD69+ CD103+ Trm cells concatenated from tumor samples (n = 49 biologically independent samples). The gray area refers to the total CD8+ T-cell subpopulation. b Mean ± SEM summary of the MFI of different activation, coinhibitory and costimulatory markers by CD127+ CD39^lo, CD127− CD39^lo, and CD127− CD39^hi Trm subpopulations from tumor samples (n = 54 biologically independent samples), as defined by flow cytometry. Numbers indicate the exact p values; one-way ANOVA with Friedman test. c Bar plots showing the frequency depicted as mean ± SEM of CD127+ CD39^lo, CD127− CD39^lo, and CD127− CD39^hi in CD8+ cells among different tissue compartments (tumor, normal breast, and peripheral blood; n = 54 biologically independent samples). Numbers indicate the exact p values for tumor vs. peripheral blood or normal tissue samples; one-way ANOVA with Friedman test. d Mean ± SEM summary of the frequency of CD127− CD39^hi Trm among total CD8+ cells in metastatic (mts; n = 12 biologically independent samples) and nonmetastatic (non-mts; n = 7 biologically independent samples) lymph nodes (LN). Numbers indicate the exact p value; Mann–Whitney test. Data were obtained in one experiment. e Flow cytometry plots from a representative patient showing the production of CD107a, IL-2, TNF, and IFN-γ by BC tumor-infiltrating CD8+ Trm subsets in response to PMA/ionomycin stimulation (STIM) in vitro. Numbers in the histograms indicate the percentage of positive cells identified by the gates. UNSTIM unstimulated control. f Mean ± SEM summary of the frequency of effector molecules expression from e (n = 6 biologically independent samples; performed in two independent experiments). Numbers indicate the exact p value; one-way ANOVA with Friedman test.