Fig. 5. Defective thrombosis in FXII_ΔPR-III reconstituted F12^−/− mice.

A Thrombus formation in vivo was monitored by intravital microscopy of mesenteric arterioles following topical application of 20% FeCl₃ in wild type (WT, top lane) and F12^–/– mice, reconstituted with buffer (second lane), FXII_fl (third lane) or FXII_ΔPR-III (bottom lane). Representative images of n = 6 experiments. Scale bar equals 50 µm. B Time to complete occlusion after injury. Each symbol represents one monitored arteriole. The number of occluded vessels during the 40 min observation period is given at the top. n = 6 mice. C Pulmonary embolism induced by intravenous infusion of collagen/epinephrine. The survival time of WT, F12^−/− or F12^−/− mice with hydrodynamic tail vein injection-mediated transgene expression of FXII_fl or FXII_ΔPR-III was monitored. Mortality was assessed in each group of mice and animals alive 30 min after the challenge, were considered survivors; Top panel: Collagen/epinephrine-challenged mice were intravenously infused with Evans blue shortly after the onset of respiratory arrest and while the heart was still beating or for survivors, at 30 min post-challenge. Lungs were harvested and perfusion was determined. Occluded lung parts remain their natural pinkish colour. The bar represents 5 mm. n = 5 mice. In B, C: ***: P < 0.001, by unpaired two-tailed Student´s t test. Each symbol represents one animal. D ETP triggered by activated platelets (25 μM ionomycin, 5 × 10⁷ platelets/mL) or buffer in FXII_fl or FXII_ΔPR-III reconstituted FXII-deficient plasma. n = 4 PRP samples. n.s.: non-significant (P > 0.05), **: P = 0.005, by unpaired two-tailed Student’s t test. Columns give means ± SD.