Figure 4. Agonistic αTIGIT induces apoptosis in all three “risky” memory T cell subsets both in the presence and absence of belatacept.

Peripheral blood T cells from healthy donors were unstimulated or stimulated ex vivo with allogeneic antigens and treated with agonistic αTIGIT or isotype control in the presence or absence of belatacept. Risky memory T cell subsets were defined as in Figure 1. Representative flow plots (A) and summary data (B) of frequencies of Caspase3/7⁺ 7-AAD⁺ apoptotic cells within risky memory T cell subsets (**, p < 0.01; *, p < 0.05; data depicted are from eight independent stimulator/responder pairs; data are representative of two independent experiments).