Fig. 2. Disruption of DWF5 results in defects in TAG and LD accumulation in leaves.

a The map position of the M1-7 mutation and structure of DWF5 (At1g50430). Markers used for mapping and the respective number of recombinants are indicated. The exons of DWF5 are shown as black boxes and introns are indicated by lines. The point mutation in the dwf5 mutant allele is indicated with a line and the T-DNA insertion with a triangle. b Growth phenotypes of 4-week-old plants. Red, blue, and white arrowheads indicate young, mature, and old leaves (for definitions, see Fig. 1), respectively. Bar = 2 cm. c Representative images of LDs (OLE1-GFP, green) in leaves of 4-week-old Col/OLE1 and dwf5-8/OLE1 plants and LDs in wild-type leaves stained by Nile Red. The experiment was repeated 4 times with similar results. Bar = 50 μm. d, e, TLC analysis of neutral lipids. Lipids were extracted from leaves of 4-week-old plants and visualized with 5% sulfuric acid by charring (d). Relative contents of TAG were quantified by Image J (e). Data are mean ± SD of three biological replicates. Different letters indicate significant difference at P < 0.05, as determined by one-way ANOVA with Tukey’s multiple comparisons test.