Fig. 3. Disruption of DWF7 or SMT2/3 but not DWF1 and DWF4 results in defects in TAG and LD accumulation in leaves.

a Representative images of LDs (OLE1-GFP, green) in young, mature, and old (for definitions, see Fig. 1) leaves of 4-week-old plants. The experiment was repeated four times with similar results. Bar = 50 μm. b, c TLC analysis of neutral lipids. Lipids were isolated from leaves of 4-week-old plants and visualized with 5% sulfuric acid by charring (b). Relative contents of TAG were quantified by Image J (c). Data are mean ± SD of three biological replicates. Different letters indicate significant differences at P < 0.05, as determined by one-way ANOVA with Tukey’s multiple comparisons test.