Fig. 4. TAG metabolism and LD accumulation in leaves of dwf mutants as revealed by oleic acid feeding.

a TAG contents in leaves before oleic acid feeding, after 0.5 mM cold oleic acid feeding for overnight and after 24 h of chase. Lipids were extracted from leaves and quantified by GC-MS. Four biological replicates were performed for dwf4-6, dwf5-8, and dwf7-4 before feeding while the others had three biological replicates. b Label incorporation into TAG in leaves after ¹⁴C-oleic acid feeding and after 24 h of chase. Lipids were extracted from leaves and separated by TLC. Radiolabeled TAGs were quantified by liquid scintillation counting. Three biological replicates were carried out for each sample. c LDs in leaves after 0.5 mM cold oleic acid feeding for overnight. LDs were stained by BODIPY red and observed with a confocal scanning laser microscope. The experiment was repeated 3 times with similar results. Bars = 20 μm (5 μm in insert). In a and b, data are mean ± SD. Different letters indicate significant differences at P < 0.05, as determined by one-way ANOVA with Tukey’s multiple comparisons test.