Figure 4.

Dendograms representing the HMC3 microglia surfaceome. (A) HMC3 cell membrane proteins represented by receptors (Tyr and Ser/Thr receptor and nonreceptor kinases; olfactory, frizzled, taste, neurotransmitter and adhesion GPCRs), transport proteins (ligand and voltage-gated ion channels, transporters of small and large molecules), cell adhesion and cell-cell (tight junctions, gap junctions, adherent junctions and desmosomes) or cell-matrix (focal adhesion) junctions, and proteins with various catalytic functions (857 proteins with two distinct peptides, out of 1314 total cell membrane proteins detected; ~18% present in multiple categories). (B) HMC3 cell membrane receptors and CD antigens. A total of 283 proteins were assigned to signaling processes triggered by immune receptors (cytokine, chemokine, PRRs, complement receptors, Fc, T-cell, B-cell, and scavenger receptors), neurotransmitter receptors, hormone and prostaglandin receptors, glycan sensors (C-type lectin and Siglecs), and other growth factor binding proteins and receptors.