Table 1.
Primers designed to amplify the entire family of known TAS2R genes in sheep
| Gene | Gene Length (bp) | Forward primer* | Reverse primer* | Amplicon length (bp)** |
|---|---|---|---|---|
| TAS2R3 | 2841 | CAGCTAACGGTCTGGAGGTC | CAGTAACAGCTTCACCGCCT | 3111 |
| TAS2R4 | 1710 | CCCAGGTTCACTTTGGTGGT | CCACAGTCCTGCTGTTCCAA | 2119 |
| TAS2R5 | 2645 | AGATTGCAGAAGGGTAAGACCA | TATCTCAAAACAGTCTCCTGACCAC | 2807 |
| TAS2R7 | 939 | GGGACCGACAACTGCATTAC | TCCTCTGGCAGTTACTGTTAAGAT | 1456 |
| TAS2R8 | 929 | GAGCTTGGAACTTTCGGAGGA | GTGCACTTTAGTAGGGGCCA | 1399 |
| TAS2R9 | 1164 | TTTGAAGTCCCTGGCCAACA | TGGTGTGAAGTGTGAACGTGA | 1500 |
| TAS2R10 (LOC101115110) | 930 | AGGCATTCAGTCTGGGTGTG | GGGAGAAACCACTGGCAAGA | 1398 |
| TAS2R10-like (LOC101122269) | 900 | TGGAGGCATCTCTGTCAAGC | GGGAGAAACCACTCCAAGGG | 1349 |
| TAS2R12 (LOC101114857) | 912 | AGCAGTGGCGACACATACAT | TGAGAGGTCATCATCACTTCAGG | 1180 |
| TAS2R16 | 928 | AGATGGCTGTGGGCAAAGAG | GGAACCTGGTCCCAAACTGG | 1126 |
| TAS2R31-like (LOC101121003) | 914 | TCCATCCCATAGTAGGGCAC | AGACACTTTTTGTTATTAGCTCAGG | 1318 |
| TAS2R38 | 1002 | GTGGAAGGGCCCATTGATGTA | AGCTTCTGCATCACCCAAGG | 1448 |
| TAS2R39 | 1092 | CACACCAGCGCATCCAAAAA | CAGCCCCGGAAATCTTGACT | 1536 |
| TAS2R40 | 1364 | TAAACCGGGACTCTTGCCCT | TGACTCTGGGTTAGTGGGGT | 1880 |
| TAS2R41 | 936 | GAGCTCAGTCACAGACACCC | TCCCAAAGGAGAAAGCCCAC | 1391 |
| TAS2R42 | 930 | TGCCGATGATGAATGCACAC | GCCTCTTCTCCCAAATACGAGT | 1519 |
| TAS2R42-like (LOC101120742) | 936 | TGCCAGCACCAATGATGAGT | GGGCATGTCCAAATGATCGTG | 1522 |
| TAS2R60 | 954 | AATTCATGGACAGGCAGCGA | TCTTTGGCCACATCAGGTCC | 1438 |
| TAS2R67 (LOC101120486) | 939 | AGTGGGCACATTCACTGCTT | TGATGCCAGTGATGCTTGCT | 1416 |
* Gene specific primers do not include Oxford Nanopore forward and reverse sequence tags.
**Amplicon length was determined from sequence data.