Table 1.

Confirmation and Selectivity of nsp14 Screening Hits.

Compound	SPR KD (µM) of Nsp14	IC50 (µM)a
		Nsp 14	G9a	BCDIN3D	ALKBH5	SETD3	METTL3-14
SS148	0.05	0.07 ± 0.006	NI	0.03 ± 0.002	NI	NI	0.9
WZ16	NA	0.19 ± 0.04	NI	1.7	NI	3.4	3.4
DS0464	1.6	1.1 ± 0.2	NI	46 ± 9	NI	NI	NI
DS0466	2.4	3.4 ± 1.4	NI	>50	NI	NI	NI
JL27-56A1	0.2	0.26 ± 0.13	NI	19	NI	23	10
Compound 8	18.9	95 ± 6	NI	NI	NI	NI	NI
SAH	0.14	0.13 ± 0.03	NA	NA	NA	NA	NA
MTTR025495	6.2	17 ± 2	NA	NA	NA	NA	NA

The screening hits were tested for binding to nsp14 by SPR and for inhibition of MTase activity of nsp14 and selected MTases by activity assays. All values are from experiments presented in Figure 3andSupplemental Figures S7, S10, S11, S12, S13, and S14. NA, not assessed; NI, no inhibition. ^aIC₅₀ values are presented as the mean ± standard deviation (n = 3).