FIGURE 3.

Increasing the number of endothelial cells does decrease the percentage of oxidative stress–positive cells (A) Three different seeding conditions of endothelial cells were used to understand if the number of endothelial cells can influence the oxidative stress in the pseudoislet. The cell ratio between α and β remained the same in all three conditions (B) The two pseudoislets (1× and 2×) with the fewest endothelial cells had a significantly lower number of oxidative stress-positive cells than the 4× pseudoislet with the induction of oxidative stress (*p < 0.03, **p < 0.008) (C, D) Looking at the α and β cells, there were significantly more oxidative stress-positive cells (**p < 0.01, ***p < 0.001) in the 4× pseudoislets. Results are expressed as mean ± SEM, and each data set includes twelve pseudoislets (n:12), and the experiment was repeated three times (N:3).