Fig. 3.

LIPUS inhibits synoviocyte proliferation and synovial hyperplasia (A) The mice were subjected to EdU assay and knee joints were harvested on day 14 after DMM surgery. S represents synovium. The dotted lines indicate the boundary of the synovium. Nuclei were stained by DAPI (blue) and EdU-positive cells appear red. Scale bar: 100 ​μm and 50 ​μm (B) Quantification of EdU-positive cells (n ​= ​3 per group) (C) Representative hematoxylin and eosin-stained images of knee joints from indicated groups of mice. Scale bar ​= ​200 ​μm (D) Synovial hyperplasia was measured by synovial thickness (n ​= ​5 per group). Statistical analysis was performed by one-way ANOVA, and multiple comparisons were performed using Tukey's test. Data are expressed as mean ​± ​SD. ∗∗p ​< ​0.01, ∗∗∗p ​< ​0.001, and ∗∗∗∗p ​< ​0.0001.