Skip to main content
. 2021 Sep 9;9:733573. doi: 10.3389/fcell.2021.733573

FIGURE 1.

FIGURE 1

Cytoskeletal remodeling in cells cultured in simulated microgravity or exposed to CYT D. (A) Staining for actin (green) and nuclei (blue) in human dermal microvascular endothelial cell (HDMEC) grown on microcarrier beads in 1g or in the Rotating Wall Vessels (RWV) for 4 and 10 days. Scale bar: 40 μm. (B) Table showing the counts of human endothelial cell from umbilical vein (HUVEC) (upper table) and HDMEC (lower table) cultured in the RWV. (C,D) Different doses of CYT D were tested on HUVEC (C) and HDMEC (D). MTT (upper panel) was performed after 24–96 or 24–72 h for HUVEC and HDMEC, respectively. Staining for actin (red) was performed at 24–96 h (HUVEC) or 24–72 h (HDMEC) in the presence of the selected dose (lower panels). Nuclear staining in blue. Scale bar: 40 μm. *P < 0.05, **P < 0.01, ***P < 0.001, and ****p < 0.0001.