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. 2021 Sep 9;9:733573. doi: 10.3389/fcell.2021.733573

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Copyright © 2021 Locatelli and Maier.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of microgravity on stress response in human dermal microvascular endothelial cell (HDMEC). (A) Western blots performed on HDMEC cultured in microgravity for 4 and 10 days. Antibodies against TXNIP, HSP70, HSP27, and P-HSP27 were used and normalized on control loading. Densitometric analysis of the blot shown is reported on the right. (B) Reactive oxygen species production in HDMEC grown for 4 and 10 days in microgravity was analyzed using 2′-7′-dichlorofluorescein diacetate. The results were expressed as % compared to the sample grown in 1g condition for 4 days. *P < 0.05 and **P < 0.01.