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. 2021 Sep 9;9:729444. doi: 10.3389/fcell.2021.729444

FIGURE 1.

FIGURE 1

The tandem WW domains of PLEKHA5 stabilize its localization along microtubules. (A–C) Scheme of the structural organization of PLEKHA5 (A), PLEKHA6 (B), and PLEKHA7 (C) showing the domains: WW (Trp-Trp) (W) in green, PH (pleckstrin homology) in red, Proline-rich (Pro-rich or Pr) in blue, coiled-coil (C) in pink. Red box indicates the structural domains analyzed. (D,E) IF analysis of the localization of GFP-tagged PLEKHA5 (D) and PLEKHA6 (E) constructs, either full-length, or a deletion lacking the WW domains (ΔWW), or the isolated N-terminal WW-PH region (WW-PH) after transfection in WT mCCD cells. (F) IF analysis of the localization of GFP-tagged chimeras of either PLEKHA6 or PLEKHA7, containing the WW domains of PLEKHA5 (P5), expressed in PLEKHA5 KO MDCK cells. α-tubulin is used as a marker for microtubules. In panels (D–F), squares correspond to enlarged areas in panels (D′–F′). Arrows show labeling, arrowheads indicate low/undetectable labeling. Junctional (junct.), fibrillar microtubules-like [(MT)], microtubules (MT) and cytoplasmic (cyt.) localizations are indicated. Scale bar = 20 μm.