TABLE 1.
Key resources.
| Reagent or resource | Source | Identifier |
| Antibodies | ||
| Rabbit polyclonal anti-PDZD11 | Citi Laboratory (Guerrera et al., 2016) | Rb29958 |
| Guinea pig polyclonal anti-PLEKHA7 | Citi Laboratory (Guerrera et al., 2016) | GP2737 |
| Mouse monoclonal anti-β-tubulin | Thermo Fisher Scientific | Cat# 32-2600, RRID: AB_2533072 |
| Mouse monoclonal anti-α-tubulin | Thermo Fisher Scientific | Cat# 32-2500, RRID: AB_2533071 |
| Guinea pig monoclonal anti-α-tubulin | Geneva Antibody Facility (Guerreiro and Meraldi (2019). Antibody Reports) | AA345 scFv-F2C |
| Rabbit polyclonal anti-GFP | Thermo Fisher Scientific | Cat# A-11122, RRID: AB_221569 |
| Mouse monoclonal anti-HA | Thermo Fisher Scientific | Cat# 32-6700, RRID: AB_2533092 |
| Mouse monoclonal anti-E-cadherin | BD Biosciences | Cat# 610181, RRID: AB_397580 |
| Rat monoclonal anti-ZO-1 | Goodenough Laboratory (Harvard Medical School) | R40.76 RRID: AB_2205518 |
| Rabbit polyclonal anti-GST | Thermo Fisher Scientific | Cat# 71-7500 RRID: AB_2533994 |
| Alexa Fluor 488-AffiniPure Donkey anti-Rabbit IgG | Jackson ImmunoResearch | Cat# 711-545-152, RRID: AB_2313584 |
| Cy3-AffiniPure Donkey anti-Mouse IgG | Jackson ImmunoResearch | Cat# 715-165-151, RRID: AB_2315777 |
| Alexa Fluor 647-AffiniPure Donkey anti-Guinea Pig IgG | Jackson ImmunoResearch | Cat# 706-605-148, RRID: AB_2340476 |
| Cy5-AffiniPure Donkey anti-Rat IgG | Jackson ImmunoResearch | Cat# 712-175-153, RRID: AB_2340672 |
| Anti-Mouse IgG (H + L), HRP conjugate | Promega | Cat# W4021, RRID: AB_430834 |
| Anti-Rabbit IgG (H + L), HRP conjugate | Promega | Cat# W4011, RRID: AB_430833 |
| Bacterial Strains | ||
| BL21-DE3 Competent cells | NEB | Cat# C2530H |
| DH5-α Competent cells | Thermo Fisher | Cat# 18265017 |
| Chemicals and Recombinant Proteins | ||
| Poly-L-Lysine solution | Sigma-Aldrich | Cat# P4707 |
| MG132 | Sigma-Aldrich | Cat# C2211 |
| GST-human PLEKHA5-PH (120-270) | Citi Laboratory (This paper) | S2090 |
| GST-human PLEKHA6-PH (120-285) | Citi Laboratory (This paper) | S2095 |
| GST-human PLEKHA7-PH (120-300) | Citi Laboratory (Guerrera et al., 2016) | S1795 |
| Critical Commercial Assays | ||
| jetOPTIMUS DNA Transfection Reagent | Polyplus | Cat# 117-15 |
| 3.5 kDa-MWCO dialysis membrane (Spectra/Por3 3,500 MWCO 18mm) | Spectrum Laboratories | Cat# 132720 |
| PIP Strips | Echelon Biosciences | Cat# P-60001 |
| Pierce Glutathione Magnetic Agarose Beads | Thermo Fisher Scientific | Cat# 78602 |
| Matrigel | BD Biosciences | Cat# 354230 |
| Protease inhibitor cocktail | Thermo Fisher Scientific | Cat# A32965 |
| Experimental Models: Cell Lines | ||
| Mouse cortical collecting duct cell line, mCCD WT N64-Tet-ON | Feraille Laboratory, Unige | N/A |
| Mouse cortical collecting duct cell line, mCCD PLEKHA7-KO N64-Tet-ON | Citi Laboratory (Guerrera et al., 2016; Shah et al., 2018) | N/A |
| Mouse cortical collecting duct cell line, mCCD PDZD11-KO N64-Tet-ON | Citi Laboratory (Guerrera et al., 2016) | N/A |
| Mouse cortical collecting duct cell line, mCCD PLEKHA6-KO N64-Tet-ON | Citi Laboratory (Sluysmans et al., 2021) | N/A |
| Mouse cortical collecting duct cell line, mCCD PLEKHA6/7-KO N64-Tet-ON | Citi Laboratory (Sluysmans et al., 2021) | N/A |
| Canine kidney proximal tubule cell line, MDCK-II WT Tet-OFF | Fanning Laboratory, U. North Carolina | N/A |
| Canine kidney proximal tubule cell line, MDCK-II PLEKHA5-KO Tet-OFF | Citi Laboratory (Sluysmans et al., 2021) | N/A |
| Canine kidney proximal tubule cell line, MDCK-II PLEKHA6-KO Tet-OFF | Citi Laboratory (Sluysmans et al., 2021) | N/A |
| Human haploid cell lines, Hap1 WT, PLEKHA7-KO | Amieva Laboratory, Standford (Popov et al., 2015) | N/A |
| Human haploid cell line, Hap1 PDZD11-KO | Citi Laboratory (Shah et al., 2018) | N/A |
| Human haploid cell line, Hap1 PLEKHA5-KO | Citi Laboratory (Sluysmans et al., 2021) | N/A |
| Human haploid cell line, Hap1 PLEKHA5/7-KO | Citi Laboratory (Sluysmans et al., 2021) | N/A |
| Recombinant DNA | ||
| pcDNA3.1(zeo +) human PDZD11-HA | Citi Laboratory (Guerrera et al., 2016) | S1766 |
| pcDNA3.1(zeo +) GFP-human PDZD11-myc | Citi Laboratory (Guerrera et al., 2016) | S1744 |
| pTRE2hyg YFP-human PLEKHA7-myc | Citi Laboratory (Paschoud et al., 2014) | S1431 |
| pTRE2hyg YFP-human PLEKHA7-Nter (1-562)-myc | Citi Laboratory (Paschoud et al., 2014) | S1432 |
| pTRE2hyg YFP-human PLEKHA7-ΔPH (1-162 + 282-1121)-myc | Citi Laboratory (Shah et al., 2018) | S1826 |
| pcDNA3.1(–) GFP-myc | Citi Laboratory (Paschoud et al., 2011) | S1166 |
| pTRE2hyg YFP-myc | Citi Laboratory (Paschoud et al., 2014) | S1210 |
| pcDNA3.1(–) GFP-human PLEKHA5-FL | Citi Laboratory (Sluysmans et al., 2021) | S2530 |
| pcDNA3.1(–) GFP-human PLEKHA5-WW-PH (1-270) | Citi Laboratory (This paper) | S2482 |
| pcDNA3.1(–) GFP-human PLEKHA5-Nter (1-504) | Citi Laboratory (This paper) | S2483 |
| pcDNA3.1(–) GFP-human PLEKHA5-Cter (410-1116) | Citi Laboratory (This paper) | S2522 |
| pcDNA3.1(–) GFP-human PLEKHA5-PH (164-270) | Citi Laboratory (This paper) | S2565 |
| pcDNA3.1(–) GFP-human PLEKHA5-ΔPH (1-169 + 271-1116) | Citi Laboratory (This paper) | S2523 |
| pcDNA3.1(–) GFP-human PLEKHA5-ΔWW (107-1116) | Citi Laboratory (This paper) | S2528 |
| pcDNA3.1(–) GFP-human PLEKHA5-FL ΔCC (1-633 + 680-1116) | Citi Laboratory (This paper) | S2654 |
| pcDNA3.1(–) GFP-human PLEKHA5-Cter ΔCC (410-633 + 680-1116) | Citi Laboratory (This paper) | S2660 |
| pcDNA3.1(–) GFP-human PLEKHA6-FL | Citi Laboratory (Sluysmans et al., 2021) | S2531 |
| pcDNA3.1(–) GFP-human PLEKHA6-WW-PH (1-284) | Citi Laboratory (This paper) | S2487 |
| pcDNA3.1(–) GFP-human PLEKHA6-Nter (1-520) | Citi Laboratory (This paper) | S2488 |
| pcDNA3.1(–) GFP-human PLEKHA6-Cter (515-1297) | Citi Laboratory (This paper) | S2525 |
| pcDNA3.1(–) GFP-human PLEKHA6-PH (158-284) | Citi Laboratory (This paper) | S2566 |
| pcDNA3.1(–) GFP-human PLEKHA6-ΔPH (1-163 + 285-1297) | Citi Laboratory (This paper) | S2526 |
| pcDNA3.1(–) GFP-human PLEKHA6-ΔWW (98-1297) | Citi Laboratory (This paper) | S2529 |
| pcDNA3.1(–) GFP-human PLEKHA6-FL ΔCC (1-724 + 774-1297) | Citi Laboratory (This paper) | S2655 |
| pcDNA3.1(–) GFP-human PLEKHA6-Cter ΔCC (515-724 + 774-1297) | Citi Laboratory (This paper) | S2661 |
| pcDNA3.1(–) GFP-human PLEKHA7-FL ΔCC (1-685 + 740-1121) | Citi Laboratory (This paper) | S2657 |
| pcDNA3.1(–) GFP-human PLEKHA7-Cter ΔCC (500-685 + 740-1121) | Citi Laboratory (This paper) | S2663 |
| pcDNA3.1(–) GFP-human PLEKHA5-WW(1-106):PLEKHA6(98-1297) (chimera) | Citi Laboratory (This paper) | S2538 |
| pcDNA3.1(–) GFP-human PLEKHA5-WW(1-106):PLEKHA7(97-1121) (chimera) | Citi Laboratory (This paper) | S2539 |
| pcDNA3.1(–) GFP-human PLEKHA6-PH(164-284):PLEKHA5(1-169/271-1116) (chimera) | Citi Laboratory (This paper) | S2532 |
| pcDNA3.1(–) GFP-human PLEKHA7-PH(165-284):PLEKHA5(1-169/271-1116) (chimera) | Citi Laboratory (This paper) | S2533 |
| pcDNA3.1(–) GFP-human PLEKHA5-PH(170-270):PLEKHA6(1-163/285-1297) (chimera) | Citi Laboratory (This paper) | S2534 |
| pcDNA3.1(–) GFP-human PLEKHA7-PH(165-284): PLEKHA6(1-163/285-1297) (chimera) | Citi Laboratory (This paper) | S2535 |
| pcDNA3.1(–) GFP-human PLEKHA5-PH(170-270):PLEKHA7(1-164/285-1121) (chimera) | Citi Laboratory (This paper) | S2536 |
| pcDNA3.1(–) GFP-human PLEKHA6-PH(164-284): PLEKHA7(1-164/285-1121) (chimera) | Citi Laboratory (This paper) | S2537 |
| pTRE2hyg YFP-human PLEKHA7-Cter (500-1121)-myc | Citi Laboratory (This paper) | S1897 |
| pTRE2hyg YFP-human PLEKHA7-PH (160-300)-myc | Citi Laboratory (This paper) | S1899 |
| Softwares and Algorithms | ||
| FIJI | Schindelin et al. (2012). Nature Methods 9(7): 676-682. | imagej.net/Fiji |
| Adobe Photoshop | N/A | adobe.com |
| Adobe Illustrator | N/A | adobe.com |
| Image Studio Lite | LI-COR | www.licor.com/bio/image-studio-lite/ |
| SnapGene | N/A | snapgene.com/ |
| GraphPad Prism 8 | N/A | graphpad.com/scientific-software/prism/ |