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. 2021 Sep 17;62(12):15. doi: 10.1167/iovs.62.12.15

Figure 2.

Figure 2.

Lysosomal dysfunction is evident in Slc4a11 KO corneal endothelial cells. (A) Wes immunoassay of v-ATPase, and Cathepsin B using Slc4a11 WT and KO MCEC whole cell lysates (± 50 nM BafA1). (B) Quantification of data from panel A, n = 3, mean ± SD. **P < 0.01, ***P < 0.001, ****P < 0.0001, ns = not significant (1-way ANOVA with Tukey's multiple comparisons test). (C) Wes immunoassay of v-ATPase, and Cathepsin B from corneal endothelium ofSlc4a11 WT and KO mice. (D) Quantification of panel C data, n = 3, mean ± SD. ***P < 0.0001, **P < 0.01 (Student's t-test). (E) Magic Red assessment of Cathepsin L activity in Slc4a11 WT and KO corneal endothelial tissue. Scale bar – 50 µm. (F) Quantification of Magic Red fluorescence, n = 5, mean ± SD. ****P < 0.0001 (Student's t-test).