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. 2021 Sep 17;62(12):15. doi: 10.1167/iovs.62.12.15

Figure 5.

Figure 5.

MitoQ improves autophagy Slc4a11 KO cells. (A) Wes immunoassay of pmTOR, mTOR, and P62 from Slc4a11 WT and KO MCEC ± MitoQ. (B) Quantification of panel A data, n = 3. (C) Western Blots of LC3b and β-actin in Slc4a11 WT and KO MCEC ± MitoQ. (D) Quantification of panel C data, n = 3. (E, F) Detection of GFP and RFP fluorescence ratio in Slc4a11 WT, KO (± MitoQ) MCEC transfected with GFP-LC3-RFP-LC3ΔG and Quantification. Scale bar – 10 µm, n = 7. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, mean ± SD (1-way ANOVA with Tukey's multiple comparisons test).