Figure 2.

The plasma levels of (A) norepinephrine (NE), (B) epinephrine (EP) and (C) dopamine (DP) after 6-OHDA treatment. Seven-day-old ICR mice were infected with MP4 by i.p. injection. At day 4 after infection, MP4-infected mice were administered i.p. with 30 μg/g/100 μL of 6-OHDA or an equivalent volume of vehicle. After one hour of 6-OHDA delivery at day 4, cardiac puncture blood collection was performed, and plasma were separated immediately by centrifugation and stored at -70°C before assay. Plasma samples were pooled from 4 to 6 mice of each data point. NE, EP, and DA were measured by competitive ELISA. Each data point of each group represented the results from a pooled plasma sample. Pre: pre-6-OHDA injection.