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. 2021 Sep 9;12:700903. doi: 10.3389/fimmu.2021.700903

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Copyright © 2021 Liao, Tsai, Wang and Chen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The flow cytometric panel for analyzing mouse PBMCs and splenocytes. Gating strategy to identify lymphocyte subsets from mouse PBMCs and splencytes. P1 and P2 were gated in FSC-A/SSC-A dot plot. From P1, CD3⁺CD4⁺ and CD3⁺ CD8⁺ cells were gated on CD3, CD4 and CD8. CD19⁺ cells were gated on CD3 and CD19. CD3^-NK1.1⁺ and CD3⁺NK1.1⁺ cells, within CD3^- and CD3⁺ cells, respectively, were defined by NK1.1 and NKp46. From P2, CD11b⁺Ly-6C⁺ and CD11b⁺Gr-1⁺ cells were gated on CD11b, Ly-6C and Gr-1.