Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 9;12:732612. doi: 10.3389/fimmu.2021.732612

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Lu, Sun, Yang, Fan, Huang, Hong, Wu, Zhou, Fang, Li, Meng, Huang and Bai

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

In vitro induction of DCs from HSPCs. (A) Workflow for the induction of HSPC-DCs. (B) Flow cytometry of pDCs (CD66b^-CD14^-CD1c^-CD123⁺CD303^-) and CD1c⁺cDCs (CD66b^-CD14^-CD1c⁺) populations after 14 days of induction from CD34⁺HSPCs. On day 14, (C) After stimulated with the TLR4 and TLR7 agonists for 2 hours, mRNA of FACS sorter purified CD1c⁺cDCs was extracted to measure gene expression using real time RT-PCR. (D) CD123⁺pDCs and CD303⁺pDCs were highly purified using FACs sorter. Then both populations were stimulated with the TLR7 and TLR9 for 2 hours, mRNA was collected to measure gene expression using real time RT-PCR. Error bars indicate mean ± SD. **P < 0.01; ***P < 0.001; ****P < 0.0001.