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. 2021 Sep 9;12:732612. doi: 10.3389/fimmu.2021.732612

Figure 6.

Figure 6

G-CSF and IFN-γ impaired the induction of HSPC-DCs subsets. (A) Flow cytometry of HSPC-DCs subset from various tested cytokines [e.g., IFN-γ (10ng/ml), IL-1β (10ng/ml), G-CSF (10ng/ml) and TNF-α (10ng/ml)] and LPS (10ng/ml), which were added into the culture system on day7. (B) The graphs showed the proliferation of HSPC-pDCs and CD1c+cDCs under various cytokines stimulation by day14. (C) The percentage of HSPC-pDCs and CD1c+ cDCs among total populations under various cytokine stimulation on day14. (D) Total HSPC-DCs were stimulated with the TLR4 and TLR7 agonists for 2 hours, mRNA was extracted to measure gene expression using real time RT-PCR. Data shown are representative of three independent experiments. Error bars indicate mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.