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. 2021 Sep 9;11:682710. doi: 10.3389/fonc.2021.682710

Figure 2.

Figure 2

SLC25A21 overexpression inhibits the cell proliferation, migration and invasion and induces the apoptosis of BCa cells in vitro. (A, B) Increased expression of SLC25A21 after transfection of the pcDNA3.1-SLC25A21 vector was confirmed in two BCa cell lines by real-time RT-PCR (A) and western blotting (B). (C) SLC25A21 overexpression inhibits the proliferation of EJ and T24 cells. (D) SLC25A21 overexpression decreased the colony formation capacity of EJ and T24 cells. (E) The migration ability of BCa cells overexpressing SLC25A21 was decreased, as indicated by the wound healing assay. (F) Matrigel invasion chamber assays showed that SLC25A21 overexpression inhibited the invasiveness of both EJ and T24 cells. Representative images (left) and quantitative analyses (right) are shown. (G) SLC25A21 overexpression induced the apoptosis rate relative to control cells. Data are presented as the mean ± SD. The results were reproducible in three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001.