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. 2021 Sep 9;8:725274. doi: 10.3389/fmolb.2021.725274

FIGURE 1.

FIGURE 1

Ionizing radiation induced induces pulmonary endothelial cell ferroptosis and increases expression of PIEZO1 protein. (A) Lipid peroxidation assessment in HULEC-5a cells, 24 h after exposure to 15-Gy of ionizing radiation (IR). Bar graphs showed relative levels of lipid peroxidation by C11-BODIPY staining in indicated cells. (B) ROS measurement in HULEC-5a cells, 24 h after IR. ROS levels were determined using H2DCFDA and analyzed using flow cytometry. (C) Western blotting analysis of ACSL4, SLC7A11, DMT1, and GPX4 protein expression in HULEC-5a cells, 6, 12, and 24 h after IR. (D) Representative transmission electron microscopy images of HULEC-5a cells after IR. Yellow arrows show mitochondria. (E) Expression of GPX4 and SLC7A11 detected by immunohistochemistry assay in lungs of mice after IR. Scale bars: 100 µm (left) and 50 µm (right). (F) Western blot analysis of PIEZO1 protein expression in HULEC-5a cells after IR. (G) Expression of PIEZO1 protein detected by immunohistochemistry assay. Scale bars: 100 µm (left) and 50 µm (right). Data were plotted as means ± SEM. n = 3 independent repeats. **, p < 0.01 vs control.