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. 2021 Sep 9;12:690973. doi: 10.3389/fpls.2021.690973

Figure 2.

Figure 2

Map-based cloning of the GmPGL2 gene. (A) Map-based cloning of the GmPGL2 allele. (i) The GmPGL2 mutation was narrowed to a 150-kb region between the InDel markers MOL2371 and MOL2411 on chromosome 5. (ii) The black arrows represent the 15 putative genes in this 150-kb genomic region; the candidate gene GmPGL2 (Glyma.05g132700) is indicated with the red arrow. (iii) Schematic diagram of the Glyma.05g132700 gene. ATG and TAG, the start and stop codons, respectively, are shown. A deletion point mutation at the 1949bp nucleotide position is shown. (B) Schematic diagram of the GmPGL2 protein. The GmPGL2 gene encodes a PPR protein of the PLS-type carrying a E+ domain. The GmPGL2 protein has 17 PPR motifs; P, L, and S represent the PPR motifs of various repeat lengths; E+ is a domain required for RNA editing. (C) Complementation of the Gmpgl2 mutant. Phenotypes of HD12, GmPGL2, and a 35S:GmPGL2 complemented Gmpgl2 plant. Scale bars=3cm.