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. 2021 Sep 9;12:690973. doi: 10.3389/fpls.2021.690973

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Copyright © 2021 Feng, Yang, Zhang, Zhiyuan, Tang, Li, Yu, Leng and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GmPGL2 is a single copy gene. (A) Phylogenetic tree of GmPGL2 and homologous GmPGL2 proteins from Arabidopsis thaliana, Medicago truncatula, Lotus corniculatus, Cajanus cajan, Cucumis sativus, and Vitis vinifera, constructed using MEGA 5 with the neighbor-joining method. (B) Synteny plot of the homologous GmPGL2 regions. Solid blue lines show the pairs of homologous genes of the two homologous chromosomes. (C) Expression of GmPGL2 in different tissues. (D) Subcellular localization of GmPGL2. GFP signals of the 3301H-GmPGL2^1-198-GFP fusion protein were localized to the chloroplast of epidermal cells of N. benthamiana leaves. Green fluorescence signals, chlorophyll auto-fluorescence signals, and a merged image are shown. Bar=10μm. (E) Transmission electron micrographs of the chloroplasts of 18-day-old HD12 and Gmpgl2 seedlings. Bar=2μm.