TABLE 4.
Spirochete counts by DF microscopy and identification of treponema species in plaque samples by nPCR in disease-associated plaque from subjects with periodontitis (group DD)
| Individual plaque sample | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | No. of samples/species |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| DF resulta | 0 | 50 | 560 | 3,100 | 860 | 1,500 | 700 | 4,400 | 900 | 250 | 51 | |
| nPCR amplification result for spirochete | ||||||||||||
| T. amylovorum | − | + | − | − | + | + | + | + | + | + | − | 7 |
| T. denticola | − | + | + | + | + | + | + | + | + | + | − | 9 |
| T. maltophilum | − | + | + | + | + | + | + | + | + | + | + | 10 |
| T. medium | + | − | − | + | + | + | + | + | + | + | − | 8 |
| T. pectinovorum | + | − | − | − | − | − | + | − | − | + | − | 3 |
| T. socranskii | − | + | + | + | + | + | + | + | + | + | − | 9 |
| T. vincentii | + | + | + | + | − | + | + | − | + | + | + | 9b |
| No. of species/sample | 3 | 5 | 4 | 5 | 5 | 6 | 7 | 5 | 6 | 7 | 2 |
a
Results are shown as 103 treponemas per sample. The mean count ± SD was (1,200 ± 1,400) × 103 treponemas (n = 10).
b
Proportion of DD subjects with T. vincentii was significantly greater than that of either HH or HD subjects (P = 0.024; Fisher exact probability test).