FIG. 5.

Association of Akt with PDE3B in intact cells. (A and B) CHO-IR/PDE3B-WT cells that had been infected with AxCAAkt-WT, AxCAAkt-AA, or AxCAAkt-K179D at an MOI of 20 PFU/cell were lysed and subjected to immunoprecipitation (IP) with polyclonal antibodies to Akt (αAkt), polyclonal antibodies to PDE3B (αPDE3B), or control serum (NRS). The immunoprecipitates were then subjected to immunoblot analysis with antibodies to HA (αHA), to Akt, or to PDE3B, as indicated. (C) CHO-IR/PDE3B-WT cells that had been infected with AxCAAkt-WT were deprived of serum for 16 h, incubated in the absence or presence of 100 nM wortmannin for 30 min, and then stimulated (or not) with 100 nM insulin for 15 min; then, association of Akt with PDE3B was assayed as in panels A and B. Data are representative of three independent experiments.