TABLE 1.

Effects of insulin and isoproterenol on the activities of various PDE3B mutants in 3T3-L1 adipocytes^a

Infection	PDE3B activityb
	Insulin (100 nM)	Isoproterenol (1 μM)
PDE3B-WT	154 ± 14	146 ± 7
PDE3B-S273A	104 ± 7	141 ± 6
PDE3B-S274A	144 ± 11	ND
PDE3B-S296A	150 ± 11	102 ± 3
PDE3B-S421A	ND	162 ± 18

^aCells that had been infected with AxCAPDE3B-WT, AxCAPDE3B-S273A, AxCAPDE3B-S274A, AxCAPDE3B-S296A, or AxCAPDE3B-S421A at an MOI of 30 PFU/cell were incubated in the absence or presence of insulin or isoproterenol, after which PDE activity was assayed in solubilized membrane fractions as described in the legend to Fig. 6B. 

^bData are expressed as percentages of the corresponding basal activity (in the absence of stimuli) and are means ± standard errors from three independent experiments. ND, not done.