Figure 1.

Mutation of ADAR1-p150’s Zα domain triggers spontaneous type I IFN responses in multiple organs

(A) Levels of the indicated mRNAs were analyzed using qRT-PCR in RNA samples extracted from tissues of WT and Adar1^mZα/mZα animals and are shown relative to Gapdh. Each dot represents an individual mouse. N.D., not detectable.

(B) Protein extracts from whole lungs from animals of the indicated genotypes were used for western blot with an α-ISG15 antibody. β-Actin served as a loading control. Each lane represents a sample from an individual mouse.

(C–E) mRNA levels of the indicated ISGs were analyzed using qRT-PCR from cultured lung fibroblasts (C), BMMCs (D), and MEFs (E) of the indicated genotypes and are shown relative to Gapdh. Each dot represents cells derived from an individual mouse.

Pooled data from biological replicates are shown with mean (A, D, and E) or mean ± SD (C) and were analyzed using unpaired t test (^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗∗p < 0.0001). See also Figure S1.