Figure 4.

Multiple hematopoietic and non-hematopoietic cell types display ISGs upregulation in Adar1^mZα/mZα lungs

(A–C) mRNA levels of the indicated ISGs were analyzed by qRT-PCR using RNA extracted from cell populations sorted from lungs of WT and Adar1^mZα/mZα mice and are shown relative to Actb. B, B cells; T, T cells; DC, dendritic cells; Mono, monocytes; Mph, macrophages; NK, natural killer cells; Neut, neutrophils; Eos, eosinophils; Total, whole lung.

(D) ISG mRNA levels were analyzed as in (A)–(C) in cell populations sorted from lungs of BM chimeric mice and are shown relative to Gapdh.

Each data point represents an individual mouse. Because of the small number of epithelial cells recovered, samples were pooled from multiple mice before RNA extraction (C). Pooled data from two (A and B) or three (D) independent experiments are shown with mean (^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, and ^∗∗∗∗p < 0.0001, unpaired t test). See also Figures S3–S5.