Skip to main content
. 2021 Sep 3;5(11):bvab146. doi: 10.1210/jendso/bvab146

Figure 1.

Figure 1.

A, Western blot analysis of rat 11β-hydroxysteroid dehydrogenase type 2 (11βHSD2) protein in cell lysates of control: CHO-rMR-pBM14-TAT3-Gluc 1 (0); CHO-rMR-pBM14-TAT3-Gluc r11HSD2 (1×), CHO-rMR-pBM14-TAT3-Gluc r11HSD2 (2×) cells, and CHO-rMR-pBM14-TAT3-Gluc r11HSD2 (3×) cells [3]. B, Corticosterone-induced mineralocorticoid receptor transactivation assessed by luciferase secretion into the media by stably transfected CHO-rMR-pBM14-TAT3-Gluc cells infected 0 (red and blue lines), 1, 2, or 3 times with a virus carrying 11βHSD2. For A and B, mean values are based on data from quadruplicate wells for each concentration of steroids from 3 separate experiments and expressed as fold over control (0), without doxycycline. Results are shown as mean ± SEM. *P less than .05 vs control. Results are shown as mean ± SEM. *P less than .05 vs the same concentration of corticosterone in control cells without doxycycline.