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11β-hydroxysteroid dehydrogenase type 2 (11βHSD2) protein measured by Western blot analysis in lysates of A, CV1-rMR MMTV-Gluc.tet-inducible-r11βHSD2, and B, CHO-rMR-pBM14-pTAT3-Gluc.tet-inducible-r11βHSD2cells incubated with graded concentrations of doxycycline for 48 hours. Kidney homogenates and kidney microsomes were used as controls. A total of 20 µg of protein was used in all lanes. Results are shown as mean ± SEM. *P less than .05 vs control (0), without doxycycline.
