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. 2021 Sep 22;40:299. doi: 10.1186/s13046-021-02090-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Implication of SRSF3 in LNCAROD-mediated regulation of PKM alternative splicing and HCC malignancy. A FISH assay determining the location of LNCAROD in HCC cells. B RT-qPCR assay of the nuclear and cytoplasmic distribution of LNCAROD in HCC cell lines. C RIP-RT-qPCR assay showing the relative enrichment of LNCAROD in anti-IgG- or anti-SRSF3-specific immunoprecipitate. D RNA pull-down assay was used to detect the interaction between LNCAROD and SRSF3. E-F Effect of SRSF3 knockdown on the LNCAROD-mediated PKM1 and PKM2 alterations in SMMC-7721 and Huh7 cells at mRNA and protein levels. Effect of SRSF3 knockdown on LNCAROD-mediated increase of Huh7 cell proliferation (G-H) (G cell viability; H colony formation), migration and invasion (I), glucose consumption (J), lactate production (K), ATP production (L), and extracellular acidification rate (ECAR) (M). Data are shown as the mean ± SD. ^*P < 0.05; ^**P < 0.01