Fig. 1.

Moderate ethanol exposure induces increased bacteria within the mesenteric lymph node (MLN) and impaired barrier function in the intestine of aged, but not young mice. A Colony forming units (CFU) from MLNs isolated from young and aged mice following 4 weeks of ethanol or vehicle treatment. n = 4 mice per group. B Intestinal fatty acid binding protein (iFABP) levels in serum, measured by ELISA. C Concentration of 4 kDa FITC-dextran in serum of young and aged mice. n = 4–8 mice per group. Data are shown as mean values ± SEM. *p < 0.05 from all other groups, #p < 0.05 from young groups by two-way ANOVA with Tukey’s multiple comparisons test