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. 2021 Sep 23;18:37. doi: 10.1186/s12979-021-00247-8

Fig. 2.

Fig. 2

Moderate ethanol fails to induce ileal Cldn1 and Reg3 gene expression in aged mice. Quantitative RT-PCR of tight junction (A-C, n = 4) and AMP (D-E, n = 6–10) gene expression in intestinal epithelial cells (IECs) isolated from young and aged mice given vehicle or ethanol. 18 s was used as the endogenous control. Data are shown as mean fold change ± SEM relative to young vehicle group. *p < 0.05 from all other groups, #p < 0.05 from young vehicle and aged ethanol by two-way ANOVA with Tukey’s multiple comparisons test