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(A) Representative chromatogram of RP-HPLC indicating globin chains analyzed. (B) Percentage of γ-globin/β-like globin protein expression in total body irradiated (DEFM and DFRM) and busulfan conditioned (ZJ32 and ZJ50) rhesus monkeys (Macaca mulatta) transplanted with CD34+ cells. The results were analyzed by RP-HPLC. The average γ-globin/β-like globin protein expression for 10 non-transplanted control animals (0.49 ± 0.19%) is represented by the red line.
