Table 3. Microsatellite allele analysis of goldfish schwannoma.
Microsatellite genotypes at four polymorphic microsatellite loci (GF1, GF17, Ca07, Ca08) in four individuals (G1-G4). The lengths of the allele(s) found at each locus in matched tumour and host tissues are indicated. An M13F tag sequence (5′-AGGAAACAGCTATGACCAT-3′) was added to the 5′ end of each forward primer; this 19 bp sequence is included in the size of the allele. Tumours (T) and matched hosts (N) share identical alleles across all loci.
G1 | G2 | G3 | G4 | ||||||
---|---|---|---|---|---|---|---|---|---|
N | T | N | T | N | T | N | T | ||
Microsatellite | GF1 | 330, 330 | 330, 330 | 330, 330 | 330, 330 | 316, 316 | 316,316 | 327,327 | 327,327 |
GF17 | 204, 204 | 204, 204 | 184, 184 | 184, 184 | 184, 184 | 184, 184 | 215,215 | 215,215 | |
Ca07 | 142, 146 | 142, 146 | 161, 161 | 161, 161 | 145, 145 | 145,145 | 137,137 | 137,137 | |
Ca08 | 201, 213 | 201, 213 | 192, 192 | 192, 192 | 192, 202 | 192, 202 | 202,247 | 202,247 |