FIGURE 3.

Next‐generation microfluidic cell devices. A,B, Schematics depicting diffusive (A) and convective (B) microfluidic cell transfection device designs. Red arrowheads denote net direction of macromolecular transport across the cell membrane. Black arrow denotes direction of fluid flow. C, Schematic and preliminary data demonstrating microfluidic delivery of a large multicistronic construct containing sgRNAs, Cas9, GFP reporter and associated promoters to patient derived iPSCs. Delivery of the same construct using Lipofectamine Stem, which we have previously used for CRISPR correction of patient iPSCs,⁸⁵ has been included for comparison